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human leukemia inhibitory factor lif blocking antibody  (R&D Systems)


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    R&D Systems human leukemia inhibitory factor lif blocking antibody
    Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; <t>LIF,</t> leukemia <t>inhibitory</t> factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.
    Human Leukemia Inhibitory Factor Lif Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human leukemia inhibitory factor lif blocking antibody/product/R&D Systems
    Average 92 stars, based on 43 article reviews
    human leukemia inhibitory factor lif blocking antibody - by Bioz Stars, 2026-03
    92/100 stars

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    1) Product Images from "Stem Cells Derived from Human Amniotic Fluid Contribute to Acute Kidney Injury Recovery"

    Article Title: Stem Cells Derived from Human Amniotic Fluid Contribute to Acute Kidney Injury Recovery

    Journal: The American Journal of Pathology

    doi: 10.2353/ajpath.2010.091245

    Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; LIF, leukemia inhibitory factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.
    Figure Legend Snippet: Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; LIF, leukemia inhibitory factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.

    Techniques Used: Incubation, Multiplex Assay, Derivative Assay, Blocking Assay, Comparison



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    R&D Systems human leukemia inhibitory factor lif blocking antibody
    Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; <t>LIF,</t> leukemia <t>inhibitory</t> factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.
    Human Leukemia Inhibitory Factor Lif Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human leukemia inhibitory factor lif blocking antibody/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    human leukemia inhibitory factor lif blocking antibody - by Bioz Stars, 2026-03
    92/100 stars
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    Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; LIF, leukemia inhibitory factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.

    Journal: The American Journal of Pathology

    Article Title: Stem Cells Derived from Human Amniotic Fluid Contribute to Acute Kidney Injury Recovery

    doi: 10.2353/ajpath.2010.091245

    Figure Lengend Snippet: Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; LIF, leukemia inhibitory factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.

    Article Snippet: 29 The cells were seeded at 4000 cells/well into 96-well plates in Dulbecco's modified Eagle's medium (Sigma-Aldrich), deprived of fetal calf serum incubated with or without 3 μg/ml of human leukemia inhibitory factor (LIF) blocking antibody (R&D Systems) in the presence of 30% conditioned medium from hAFSCs or MSCs.

    Techniques: Incubation, Multiplex Assay, Derivative Assay, Blocking Assay, Comparison